Fractionation of yeast amino acid-acceptor ribonucleic acids by counter-current distribution.

Considerable interest has developed in recent years in the separation of the amino acid-specific “soluble’‘-fraction ribonucleic acids because of the probable role of these RNAs in protein biosynthesis (l-5) and because the elucidation of the structures of the different amino acid-specific RNAs may lead to an understanding of the mechanism of information transfer by nucleic acids. Preliminary studies of the separation of these RNAs by countercurrent distribution have been reported (6-11). The present paper describes in detail countercurrent distribution studies of yeast amino acid-acceptor RNAs.